2016•International Journal of SurgeryRequires access
Expression of p38MAPK in human MCF-7 breast cancer cells treated by dexamethasone
Changyou Shan, Zhijun Dai, Tiantian Kong, Huafeng Kang, Haitao Guan, Weili Min, Xiaobin Ma, Baofeng Wang, Xi-Jing Wang
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Abstract
Objective
To investigate the effect of Dexamethasone(Dex) on the proliferation and apoptosis of human breast cancer cell line MCF-7.
Methods
Under different Dex concentrations, the human breast cancer cell line MCF-7 cells were observed by inverted microscope; the growth inhibitory rates were detected by MTT test; and apoptosis was determined by Annexin V-FITC staining flow cytometry(FCM); the expression of p38MAPK protein was detected by immunocytochemistry; and the expression of p38 gene was detected by semi-quantitative PCR.
Results
Dex could significantly inhibit the growth of MCF-7 in a dose and time dependent manner, and 10-6 mol/L of Dex could induce apoptosis obviously. The expression of p38 mRNA and p38 MAPK is significantly increased in MCF-7 when treated by Dex.
Conclusions
Dexamethasone can inhibit cell proliferation, induce apoptosis in MCF-7 cell, Which is time and concentration-dependent, with an up-regulating expression of p38.
Key words:
Dexamethasone; Mitogen-activated protein kinase; Breast neoplasms; Apoptosis