Optimization of processing parameters for extraction of total alkaloids of Rhizoma corydalisin Rhizoma corydalisDispensing Granules by using Box-Behnken experimental design

Objective To optimize the processing parameters and apply for the extraction of the total alkaloids of Rhizoma corydalis in Rhizoma corydalis Dispensing Granules.Method Based on the results of single-factor-a-time-technique,a response surface methodology was employed,with the extraction duration,the ratio of water to sample,the extraction times as the independent variables.The response variable was the extraction rate of total alkaloids of Rhizoma corydalis.Result According to regression equation to determine,the best extraction conditions for the extraction duration were as follows:100 min,the ratio of water to sample 12.5∶1(mL·g-1),extraction three times.Conclusion Under the process conditions,the total alkaloids extraction rate of Rhizoma corydalis is 1.94%,which was in consistency with the theoretic value and had some practical value.