Optimization of extraction process for Corydalis Rhizoma and correlation analysis on its chemical constituents and pharmacodynamic index

Objective To evaluate the inhibitory effect of Corydalis Rhizoma on hepatoma carcinoma cell,the extraction process of two alkaloids in Corydalis Rhizoma was optimized with orthogonal design and the coefficient correlation for the two alkaloids and pharmacodynamic index was analyzed.Methods The extraction process of the two alkaloids was optimized using orthogonal design with four indexes which included the extraction rates of tetrahydropulmatine and biflorine,MTT inhibitory ratio of SMMC-7721 cell,and the rate of extractum extracted.Pearson correlations were used for analysis on coefficient correlation with SPSS 13.0 software.Results The optimal conditions were to reflux extraction with 70% alcohol as much as six times of the medicine amount for once in 2 h.The highest contents for both tetrahydropulmatine and biflorine and the highest inhibitory rate of SMMC-7721 cell were demonstrated.Conclusion The rapid and simple method could provide the experimental support for the quantitative determination of two alkaloids in Corydalis Rhizoma.