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2009•Acta Agriculturae Boreali-SinicaRequires access

Study on Transformation Efficiency by Agrobacterium-mediated in Tomato

Y. C. Ting

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Abstract

Tomato(Lycopersicon esculentum Mill)cultivars,Texuan-28,Taiyuan 823 were used as the materials,and cotyledon and hypocotyls were cultured to optimize the transformation system via Agrobacterium.The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 d prior to infect with Agrobacterium(OD600=0.3)for 6 min growing on the culture medium of MS+2.0 mg/L 6-BA+0.5 mg/L IAA.It was proved that the target NPTII gene had been integrated into the genome of regenerated plants by PCR analysis.

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What this paper is about

Tomato(Lycopersicon esculentum Mill)cultivars,Texuan-28,Taiyuan 823 were used as the materials,and cotyledon and hypocotyls were cultured to optimize the transformation system via Agrobacterium.The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 d prior to infect with Agrobacterium(OD600=0.3)for 6 min growing on the culture medium of MS+2.0 mg/L 6-BA+0.5 mg/L IAA.It was proved that the target NPTII gene had been integrated into the genome of regenerated plants by PCR analysis.

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Available abstract

Tomato(Lycopersicon esculentum Mill)cultivars,Texuan-28,Taiyuan 823 were used as the materials,and cotyledon and hypocotyls were cultured to optimize the transformation system via Agrobacterium.The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 d prior to infect with Agrobacterium(OD600=0.3)for 6 min growing on the culture medium of MS+2.0 mg/L 6-BA+0.5 mg/L IAA.It was proved that the target NPTII gene had been integrated into the genome of regenerated plants by PCR analysis.

Key concepts: Lycopersicon, Agrobacterium, Cotyledon, Transformation (genetics), Explant culture, Hypocotyl, Cultivar, Biology

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