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2010•Journal of Inner Mongolia University for the NationalitiesRequires access

Study on Transformation Efficiency by Agrobacterium-mediated in Sunflower

Yang Shichun

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Abstract

Sunflower were used as the materials,and cotyledonary nodes of sunflower were cultured to optimize the transformation system via Agrobacterium.The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 d prior to infect with Agrobacterium(OD600 =0.6) for 8 min growing on the culture medium of MS+1.2 mg/L6-BA+ 0.03mg/LIAA.It was proved that the target LycB gene had been integrated into the genome of regenerated plants by PCR analysis.

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What this paper is about

Sunflower were used as the materials,and cotyledonary nodes of sunflower were cultured to optimize the transformation system via Agrobacterium.The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 d prior to infect with Agrobacterium(OD600 =0.6) for 8 min growing on the culture medium of MS+1.2 mg/L6-BA+ 0.03mg/LIAA.It was proved that the target LycB gene had been integrated into the genome of regenerated plants by PCR analysis.

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Available abstract

Sunflower were used as the materials,and cotyledonary nodes of sunflower were cultured to optimize the transformation system via Agrobacterium.The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 d prior to infect with Agrobacterium(OD600 =0.6) for 8 min growing on the culture medium of MS+1.2 mg/L6-BA+ 0.03mg/LIAA.It was proved that the target LycB gene had been integrated into the genome of regenerated plants by PCR analysis.

Key concepts: Sunflower, Agrobacterium, Transformation (genetics), Explant culture, Biology, Transformation efficiency, Horticulture, Botany

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