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TARGETED INHIBITION OF RNAi ON HBV REPLICATION AND HBeAg EXPRESSION

Chaoying Liu

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Abstract

Objective To observe the inhibition of RNA interference(RNAi) targeting the HBV core gene on HBV replication.MethodsNt2021-2049 of HBV genome was selected as the target sequence,the respective sense and antisense oligonucleotide were synthesized,annealed and was cloned into the shRNA expressing plasmid,which was then cotransfected the HepG2 cell with the HBV plasmid.Afterwards,the inhibition on HBV in the HepG2 cell was observed.ResultsReplication of HBV and expression of HBeAg were found inhibited significantly.ConclusionThe chosen target region is effective in inhibiting HBV from replication by RNAi.

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What this paper is about

Objective To observe the inhibition of RNA interference(RNAi) targeting the HBV core gene on HBV replication.MethodsNt2021-2049 of HBV genome was selected as the target sequence,the respective sense and antisense oligonucleotide were synthesized,annealed and was cloned into the shRNA expressing plasmid,which was then cotransfected the HepG2 cell with the HBV plasmid.Afterwards,the inhibition on HBV in the HepG2 cell was observed.ResultsReplication of HBV and expression of HBeAg were found inhibited significantly.ConclusionThe chosen target region is effective in inhibiting HBV from replication by RNAi.

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Available abstract

Objective To observe the inhibition of RNA interference(RNAi) targeting the HBV core gene on HBV replication.MethodsNt2021-2049 of HBV genome was selected as the target sequence,the respective sense and antisense oligonucleotide were synthesized,annealed and was cloned into the shRNA expressing plasmid,which was then cotransfected the HepG2 cell with the HBV plasmid.Afterwards,the inhibition on HBV in the HepG2 cell was observed.ResultsReplication of HBV and expression of HBeAg were found inhibited significantly.ConclusionThe chosen target region is effective in inhibiting HBV from replication by RNAi.

Key concepts: RNA interference, HBeAg, Small hairpin RNA, Plasmid, Virology, Biology, Hepatitis B virus, Molecular biology

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