Effect of antioxidant PDTC on the proliferation and apoptosis of human cervical carcinoma SiHa cell lines in vitro

Objective To investigate the effect of antioxidant pyrrolidinedithiocarbamate(PDTC) on human cervical carcinoma SiHa cell lines.Methods Human cervical carcinoma SiHa cell lines which were cultured in vitro were treated with different concentrations of PDTC for different durations.Then the proliferation of SiHa cells were detected by WST-8 assay,the apoptosis was assessed by Hoechst 33258 staining and Annexin Ⅴ-FITC /PI double-labeled apoptosis detection kit,and the activity of nuclear factor-κB was determined by electrophoretic mobility shift assay(EMSA).Results PDTC could significantly inhibit the proliferation and induce the apoptosis of SiHa cells in a concentration-dependent manner and in partly time-dependent manner.Apoptosis could be observed by Hoechst 33258 staining.After being treated with 20,50,and 100 μmol /L PDTC for 48 hours,the early apoptosis rates of SiHa cells were(7.00 ± 1.50)%,(14.22 ± 4.02)%,and(34.39 ± 4.34)%,respectively(P 0.01).The late apoptosis rate of SiHa cells treated with 100 μmol /L PDTC was(12.16 ± 1.54)%,which was significantly higher than those in other groups(P 0.01).Furthermore,EMSA revealed that PDTC could significantly and effectively decrease constitutive NF-κB DNA binding activity at max for 4 h and in a dose-dependent manner.The inhibitory effects showed a linear positive correlation to the proliferation inhibitory rate at 24 h(r = 0.931,P = 0.035) and to a guadratic positive corelation the early apoptosis rate at 48 h(r = 1.000,P = 0.011).Conclusion PDTC can inhibit NF-κB signaling pathway,so as to induce the apoptosis of SiHa cells and finally inhibit the proliferation of them.