Inhibition of Sinogen on HBV Replication of HepG2. 2. 15 Cell line in Vitro

HepG2. 2. 15 cell line, transfected with HBV DN,was used as an experiment mod- el. MTT assay was employed to detect the growth inhibition of 2. 2.15 cells treated by Sinogen. The change of the cell cycle was measured by flow cytometry technique (FCM). The effects of Sinogen on the secret HBeAg, HBsAg, as well as HBVDNA, were assayed by ELISA method and PCR-Hybridization, respectively. The results showed that the growth inhibition of 2. 2. 15 cells was observed after treatment of Sinogen. The inhibition rate was dose-dependent and reached maximum when the treated concentration reached 3 200 IU/mL. Its IC50 was 4 078. 5 IU/mL. FCM assay showed that 2.2.15 cell's cycle was arrested on G1 and G2-M phage. The HBsAg secreting was inhibited by 90%, in the meantime, HBeAg and HBV DNA were partially inhibited by Sinogen. Their inhibition rates were 50% and 60%, respectively.