Effects on Experimental Colon Cancer by Combination of Endostatin Transfected Oral Powder Preparation (ETB-2) and 5-Fu or Recombinant IFN-α 2a

Objective To investigate the effects on experimental colon cancer by combination of ETB-2(E) and 5-Fu(F) or recombinant IFN-α 2a(I) in BALB/c nude mice,and its mechanism.Methods The human colon cancer line LoVo was implanted subcutaneously in BALB/c nude mice.Then the mice were allocated into 6 groups:the control group(n=6),E(4.5 g/kg·d~(-1),n=6),F(every time 140 mg/kg,n=6),I(every time 1.5 million IU/kg,n=6),E+F(n=6) and E+I(n=6),the dosage similar to above.The control groups were given saline water,E was given by drenching 7 days after being implanted,F and I were given by intraperitoneally injection 10,12 and 14 days after being implanted.The drenching was conducted for successive 14 days,then the mice were sacrificed on the 22nd day and the rate of tumor inhibition was calculated.The pathologic changes were observed by ([CM(155mm]conventional pathological method,the microvessel density(MVD),proliferating cell nuclear antigen)