[The inhibitory effect of antisense adenovirus vector of telomerase on telomerase activity in breast cancer cells MCF-7].

OBJECTIVE: To inhibit telomerase activity in breast cancer cells MCF-7 by antisense ribonucleotide technique. METHODS: Recombinant adenovirus was chosen as gene delivery vector to transfer and express the antisense RNA component of telomerase in tumor cells. The recombinant adenovirus of anti-telomerase RNA was obtained through gene recombination and co-transfection with lipofectin. The integrated potential of antisense virus was certified by Southern blot and the telomerase activity of MCF-7 was analysed by TRAP-silver staining. RESULTS: The MCF-7 cell line is a typical model for malignant breast cancer. In this study, the telomerase activity of MCF-7 was significantly inhibited after antisense virus infection when compared with the control cell lines. CONCLUSION: The introduction of antisense cDNA of telomerase RNA can significantly inhibit the activity of telomerase in MCF-7 cells.