Isolation,Identification,Serotype and Virulence of Vibrio parahaemolyticus Isolated from the Aquatic Products in Shanghai Markets

Vibrio parahaemolyticus(Vp) has been considered as one of the most threatening foodborne bacterial pathogens.The aim of this study is to uncover the situation of contamination of Vibrio parahaemolyticus which isolated from the shellfish in the retail market of Shanghai,and provide some guidelines for Vp prevention.For this,we carried out the international method GB/T 4789.7-2008 to isolate bacteria and analyze its physiological and biochemical characteristics.Additionally,some primers for tlh specific genes and virulence genes(tdh,trh) recommended by FDA were used for the PCR analyze.The result shows that 45 strains of Vibrio parahaemolyticus were isolated from 161 samples which come from the shellfish market of Shanghai The average detection rate is 27.95% Based on the PCR detection of tdh and trh gene,there were only 7 tdh gene found among the 45 strains,but none of them contain the trh gene.45 strains of Vp consisted of 7 serum groups:O1 2.2%(1/45),O3 24.4%(11/45),O4 15.6%(7/45),O5 20.0%(9/45),O9 4.4%(2/45),O10 8.9%(4/45),O11 26.7%(12/45).