2012•Zhongguo redai yixueRequires access
Detection of Vibrio parahaemolyticus strains isolated from food in Shenzhen city in 2010
WU Ping-fang
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Abstract
Objective To study the serotype,distribution and molecular characteristics of Vibrio parahaemolyticus isolated from food in Shenzhen city in 2010 and provide evidence for the prevention and control of diarrheal diseases caused by Vibrio parahaemolyticus.Methods 159 Vibrio parahaemolyticus strains isolated from food were sero-typed,and 14 Vibrio parahaemolyticus strains classified in group O4 were analyzed by pulsed-field gel electrophoresis(PFGE),and all of three virulence genes(TLH,TDH and TRH)were detected by real-time PCR.Results 159 Vibrio parahaemolyticus strains belonged to 46 serotypes,in which O2:K28 accounted for 6.67% being the predonimating seroypes.The similarity of 14 Vibrio parahaemolyticus strains(O4)was less than 80% and with significant difference in strain maps.And the virulence genes were TLH+,TDH-,TRH-.Conclusion The patterns of Vibrio parahaemolyticus isolated from food are scattered,and food has serious cross-contamination.Food supervision should be strengthened to guarantee the food safety.