Effect of Topiramate on Cognitive Function and Serotonin,Acetylcholine Esterase in Hippocampus of Pentetazole-Induced Epileptic Rats

Objective To explore the effect of Topiramate(TPM)on cognitive function and the content of serotonin(5-HT)and acetylcholine esterase(AChE)activity in hippocampus of Pentetazole(PTZ)-induced epileptic rats.Methods Sixty healthy rats of 28 days were randomly divided into 6 groups:blank control group,common dose TPM(0.048 g/kg)control group(TPM A group),large dose TPM(0.036 g/kg)control group(TPM B group),PTZ(0.035 g/kg)kindled group(PTZ group),common dose TPM(0.018 g/kg)treated group(PTZ and TPM A group),and large dose TPM(0.036 g/kg)treated group(PTZ and TPM B group).Every group had 10 rats.PTZ and TPM were consecutively used for 4 weeks.Every group was subjected to Y-maze to test the learning and memory after the kindled group reaching the criterion of kindling.The activity of AChE in hippocampus was measured by chemicolorimetric method,while the expression of 5-HT in hippocampus was determined by immunohistochemical method.Results Compared TPM-control A and B groups with blank group,comped TPM-control B group with TPM-control A group,and compared PTZ kindled group with blank group,the learning and memory ability were obviously worse(Pa0.001),the number of 5-HT positive neuron cells in immunoreactivity was remarkably smaller(Pa0.001),and the AChE activity in hippocampus was obviously higher(Pa0.001).Compared TPM-treated A and B groups with PTZ kindled group,compared TPM-treated A group with TPM-control A group,and compared TPM-treated B group with TPM-control B group,the learning and memory ability were obviously better(Pa0.001),and the number of 5-HT positive neuron cells in immunoreactivity was noticably larger(Pa0.001),and the AChE activity in hippocampus was clearly lower(Pa0.001).Conclusions TPM is harmful to cognitive function,and the degree of cognitive functional impairment may be related to the does of drug.The cognitive functional impairment caused by TPM may be related with the decrease of 5-HT and the increase of AChE activity in rats hippocampus.TPM may indirectly improve cognitive function by controlling the epilepsy seizure.