Separation and determination of Kaempferol、 Quercetin,and Chlorogenic acid in Folium PyrrosiaeChinese traditional medicine by micellar electrokinetic Capillary Chromatography

Micellar electrokinetic capillary chromatography was applied to the simultaneous determination of kaempferol,quercetin and chlorogenic acid in Folium Pyrrosiae.The detection limit of three components was 0.2×10-6 g/mL(chlorogenic acid),0.4×10-6 g/mL(quercetin) and 0.5×10-6 g/mL(kaempferol)separately.The regression equation showed good linearity with R2=0.9991,R2=0.9991 and R2=0.9994 for chlorogenic acid,quercetin and kaempferol,respectively.In conclusion,this method is a simple,rapid,precise and reliable technique.Contents of chlorogenic acid,quercetin and kaempferol in the crude drug of Folium Pyrrosiae could be easily determined by the proposed method with satisfactory results.