Effects of fluoride on proliferation and differentiation of osteoblast in rats

Objective Effect of fluoride on proliferation and differentiation of osteoblast in rats was studied in vitro.Methods Various concentration of sodium fluoride (NaF) were added to the osteoblasts.The proliferative response to NaF was determined by the percents of reduced Alamar Blue.The Activity of alkaline phosphatase(ALP) was measured by Elisa method.Results NaF increased the proliferation of rat osteoblast at low doses (100?μmol/L-1?mmol/L),whereas at high doses (≥2?mmol/L) it decreased the proliferation;ALP activity was increased at 10-50?μmol/L,and decreased at ≥100?μmol/L.Conclusion Fluoride had two-phase effects.At low doses it promoted osteoblast's proliferation and differentiation while at high doses it had a negative effect.