Effects of Bushen Yangxue Prescription on Expression of Bcl-2 and Bax Protein in Mice Ovaries with Immune Premature Ovarian Failure

Objective: To study the effect of Bushen Yangxue prescription,on expression of B-cell lymphoma / leukemia-2( Bcl-2) and Bcl-2 associated X protein( Bax) protein in ovaries of mice with immune premature ovarian failure( POF). Method: BALB / c female mice model of POF was established by multiple sites subcutaneous injection of zona pellucida 3 of mice,and then the modeled mice were treated with low,middle and high dosage Bushen Yangxue prescription,and positive control medicine of Estradio Valerate. Follicle-stimulating( FSH),17-estradiol( E 2),lactogenic hormone( LH) were detected by radioimmunoassay method. Expression levels of Bcl-2 and Bax protein in mice ovarian were detected by western blot method. Result: Compared to Estradio Valerate group,FSH,E 2,LH of low,middle and high dosage Bushen Yangxue prescription group were improved. Compared to the black control group,the expression of Bcl-2 protein of model group decreased obviously and the expression of Bax protein of model group increased obviously,Bcl-2 / Bax ratio of model group decreased( P 0. 05). Compared to the model group,the expression of Bcl-2 protein of high-dosage Bushen Yangxue prescription group and Estradio Valerate group increased obviously( P 0. 01); the expression of Bcl-2 protein of low and middle dosage Bushen Yangxue prescription group increased obviously( P 0. 05),the expression of Bax protein of different dosage decreased obviously( P 0. 05). All the data showed a dose-response relationship to some degree in expression of Bcl-2 protein within low,middle and high dosage Bushen Yangxue prescription group. Conclusion: It is indicated that rapid apoptosis of follicle may result in premature ovarian failure,and Bushen Yangxue prescription can improve the function of ovaries by up-regulating the expression of Bcl-2 protein and down-regulating the expression of Bax protein in ovarian.