Effect of Obstructive Jaundice of Different Degrees on the Liver Regeneration in Rats of Post-hepatectomy

Objective To explore the effect of obstructive jaundice of different degrees on the liver regeneration in rats of post-hepatectomy. Methods Rat models with obstructive jaundice were set up and used for evaluating bilirubin in different periods. A group,B group,C group and D group were established respectively in accordance with the level of bilirubin at 0,1,3 and 7 days. The rats then received hepatectomy(about 70% of total liver). Mortality was thus followed. The observance of liver function was conducted at postoperative 0,12,24,48,72 and 168 hours,so were the study of PCNA index with immunohistological analysis,and HGF mRNA expression in remnant liver tissues. The ratio of the remnant liver's weight against body weight at 7 days after operation was also collected. Results After hepatectomy,compared with C group and D group,the mortality,serum liver function,PCNA index with immunohistological analysis,HGF mRNA expression in remnant liver tissues and the ratio of the remnant liver's weight against body weight were significantly different (P0.05 ) in rats of B group. Conclusion Hyperbilirubinemia caused by biliary obstruction before operation obviously inhibits the liver regeneration of post-hepatectomy. Hepatectomy is considered relatively safe only when a relatively low level of preoperative bilirubin and short-termed obstruction are achieved.