Apoptosis inducing effect of TRAIL on bladder cancer cell line EJ

Objective To study the apoptosis inducing effect of TRAIL on EJ cells.Methods EJ cell line was incubated with different concentrations of TRAIL for 4-24 h.Annexin V fluorescence staining,DNA laddering and flow cytometry were employed to detect the accurate stage of the EJ apoptosis and the corresponding degree.Results Apoptosis could be observed as early as 4 h after EJ cells were incubated with rhTRAIL through Annexin V fluorescence staining.DNA laddering revealed the higher the concentration of rhTRAIL,the more the apoptotic cells.Flow cytometry indicated TRAIL could induce apoptosis in time-dependent manner.Conclusion Apoptosis can be induced by TRAIL on EJ cell line.TRAIL can become the possible therapy for bladder cancer.