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The growth inhibition and induction of apoptosis in rat C 6 glioma cells by TNF-α

Chao Niu

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Abstract

AIM To study the effect of TNF α on the biological activity of glioma cells. METHODS The effect of the proliferation of TNF α on rat C 6 glioma cells was determined using MTT assay. The induced apoptosis was studied by electron microscopy and DNA electrophoresis, the expression bcl 2 of rat C 6 glioma cells was analyzed by immunocyto chemistry assay. RESULTS The growth rate and proliferation activity of C 6 cells were inhibited and the apoptosis was induced by TNF α, but the expression of bcl 2 was not influenced. CONCLUSION TNF α may inhibit the proliferation and induce apoptosis but do not affect bcl 2 expression of malignant glioma cells.

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What this paper is about

AIM To study the effect of TNF α on the biological activity of glioma cells. METHODS The effect of the proliferation of TNF α on rat C 6 glioma cells was determined using MTT assay. The induced apoptosis was studied by electron microscopy and DNA electrophoresis, the expression bcl 2 of rat C 6 glioma cells was analyzed by immunocyto chemistry assay. RESULTS The growth rate and proliferation activity of C 6 cells were inhibited and the apoptosis was induced by TNF α, but the expression of bcl 2 was not influenced. CONCLUSION TNF α may inhibit the proliferation and induce apoptosis but do not affect bcl 2 expression of malignant glioma cells.

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Available abstract

AIM To study the effect of TNF α on the biological activity of glioma cells. METHODS The effect of the proliferation of TNF α on rat C 6 glioma cells was determined using MTT assay. The induced apoptosis was studied by electron microscopy and DNA electrophoresis, the expression bcl 2 of rat C 6 glioma cells was analyzed by immunocyto chemistry assay. RESULTS The growth rate and proliferation activity of C 6 cells were inhibited and the apoptosis was induced by TNF α, but the expression of bcl 2 was not influenced. CONCLUSION TNF α may inhibit the proliferation and induce apoptosis but do not affect bcl 2 expression of malignant glioma cells.

Key concepts: Apoptosis, Glioma, MTT assay, Tumor necrosis factor alpha, Cell growth, Chemistry, Molecular biology, Growth inhibition

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