Inhibition of osteoblast apoptosis using Compound Xian Zhen Decoction

Objective To test the capability of the Compound Xian Zhen Decoction in different concentrations to inhibit osteoblast apoptosis induced by tumor necrosis factor-alpha (TNF-α) . Methods After induction of apoptosis of osteoblasts cultured in vitro, the Compound Xian Zhen Decoction in different concentrations was added to the culture medium, the rate of osteoblast apoptosis was tested by flow cycometry. Results Comparied with the model group and the control group, the groups treated with the Compound Xian Zhen Decoction in concentrations of 1 μg/ml and 500 μg/ml manifested reducied rates of osteoblast apoptosis with significant difference; the drug in the concentrations of 1 μg/ml had higher effect.The drugs may affect G0-G1 stages and S stage of cell cycle to inhibit the osteoblast apoptosis, and the drug in the concentrations of 1 μg/ml increased the quantity of osteoblasts in 67-M stages. Conclusions The Compound Xian Zhen Decoction can inhibit osteoblast apoptosis induced by TNF-a and has best effect in low concentration (1 μg/ml).