Effect of Qianggubao decoction(强骨宝方)on osteoblast cultured in vitro

Objective: To study the effect of Qianggubao decoction(强骨宝方)extract of different concentration on proliferation,differentiation and mineralization of osteoblast(OB)cultured in vitro.Methods: The OB was isolated from the skull of two SD rats which were 1 to 2 days after born by means of Trypsin-type Ⅱcollagenase enzymatic digestion.After identified and subcultured,the OB was divided into control group and 4 experimental groups.Different concentration of 100,50,10 and 5 μg/ml Qianggubao decoction(强骨宝方)extract were added to the OB in 4 experimental groups.The OB in control group was cultured with normal nutrient medium without Qianggubao decoction(强骨宝方)extract.The effects of Qianggubao decoction(强骨宝方)extract on the proliferation,differentition and mineralization of osteoblasts was observed by MTT analysis,ALP analysis and mineralization formation respectively.Results:The Qianggubao decoction(强骨宝方)extract of 100,50 and 10 μg/ml promoted proliferation,differentiation and mineralization of osteoblast,in which the Qianggubao decoction(强骨宝方)extract of 100 μg/ml and 50 μg/ml had the better effects.Conclusion: The Qianggubao decoction(强骨宝方)extract of pH=7.0,50 μg/ml which contains 2 grams crude drug in 1ml is most suitable for proliferation,differentition and mineralization of osteoblastic cultured in vitro.