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Cloning and identification of the gene encoding mouse CD1d2

Xiaodong Liang

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Abstract

Objective:To clone the gene encoding mouse CD1d2(mCD1d2). Methods:Total RNA was extracted from the thymus tissue, mCD1d2 was amplified by RT-PCR and was cloned into pGEM-T vector,which was subsequently tranfomed into E.Coli DH5α,the insertion of gene and the recombinant plasmid were identified by restrict enzyme digestion and DNA sequencing. Results:A specific DNA band was seen by agrose electrophoresis,the result of the DNA sequencing showed that the mCD1d2 coding regeion gene of 1008 bp was obtained. Conclusion:The mCD1d2 coding regeion gene cloned successfully,which laid the foundations for further research on the function of mouse CD1d2.

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What this paper is about

Objective:To clone the gene encoding mouse CD1d2(mCD1d2). Methods:Total RNA was extracted from the thymus tissue, mCD1d2 was amplified by RT-PCR and was cloned into pGEM-T vector,which was subsequently tranfomed into E.Coli DH5α,the insertion of gene and the recombinant plasmid were identified by restrict enzyme digestion and DNA sequencing. Results:A specific DNA band was seen by agrose electrophoresis,the result of the DNA sequencing showed that the mCD1d2 coding regeion gene of 1008 bp was obtained. Conclusion:The mCD1d2 coding regeion gene cloned successfully,which laid the foundations for further research on the function of mouse CD1d2.

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Available abstract

Objective:To clone the gene encoding mouse CD1d2(mCD1d2). Methods:Total RNA was extracted from the thymus tissue, mCD1d2 was amplified by RT-PCR and was cloned into pGEM-T vector,which was subsequently tranfomed into E.Coli DH5α,the insertion of gene and the recombinant plasmid were identified by restrict enzyme digestion and DNA sequencing. Results:A specific DNA band was seen by agrose electrophoresis,the result of the DNA sequencing showed that the mCD1d2 coding regeion gene of 1008 bp was obtained. Conclusion:The mCD1d2 coding regeion gene cloned successfully,which laid the foundations for further research on the function of mouse CD1d2.

Key concepts: Gene, Cloning (programming), Molecular biology, Recombinant DNA, DNA sequencing, DNA, Coding region, clone (Java method)

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