CLONING AND IDENTIFICATION OF THE GENE CODING FOR MOUSE CD1D1

OBJECTIVE:To clone the gene encoding mouse CD1d1(mCD1d1).METHOD:Total RNA was extracted from the tissue of mouse small intestine.CD1d1 gene was amplified by RT-PCR,and was cloned into pGEM-T vector,which was subsequently transformed into E.Coli DH5α.The insertion of gene and the recombinant plasmid were identified by restrict enzyme digestion and DNA sequencing.RESULTS:A specific DNA band was seen by agarose electrophoresis.The mCD1d1 gene was inserted into the pGEM-T vector.The result of the DNA sequence showed that a gene fragment of 1011bp was achieved.CONCLUSION:The gene encoding mCD1d1 was cloned successfully,which lays the foundation for further preparation of the recombinant mCD1d1 and its antibody.