Study on Extraction Technology of Flavonoids from Loquat Leaves by Two Kinds of Aqueous Two-Phase Systems

Using loquat leaves as material,the flavonoids was extracted in aqueous two-phase system.The effects of the conditions,such as the amount of substances,the solid-liquid ratio(g∶mL),the pH value,the extraction temperature,the extraction time,on the extraction efficiency were studied in PEG-(NH4)2SO4system and ethanol-(NH4)2SO4system.And the extraction efficiency of the two kinds of aqueous two-phase systems were compared.The results showed that when the amount of PEG was 2.0g,the amount of ammonium sulfate was 1.5g,the solid-liquid ratio was 0.3∶10(g∶mL),the pH value was 9,the extraction temperature was 40℃,the extraction time was 1.5hin the PEG-(NH4)2SO4system;when the amount of anhydrous ethanol was 4.0mL,the amount of ammonium sulfate was 1.8g,the solid-liquid ratio was 0.2∶10(g∶mL),the extraction temperature was 40℃,the extraction time was 1.0hin the ethanol-(NH4)2SO4system,the extraction rate reached the maximum of 5.80%,7.49%,respectively.The result showed that the extraction efficiency of ethanol-(NH4)2SO4system was better than that of PEG-(NH4)2SO4system.