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2013•China Animal Husbandry & Veterinary MedicineRequires access

Cloning and Sequence Analysis of TLR5M Full-length cDNA in Common Carp Peripheral Blood Leukocytes

Zhe Sun

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Abstract

In this paper,we have isolated,sequenced and characterized the full-length cDNA of TLR5M gene from common carp by the methods of rapid amplification of cDNA ends( RACE) on the basis of its EST sequences. The results showed that the TLR5M gene was 3182 bp in length,vthe gene consists of a 38 bp 5' untranslated region( UTR),486 bp 3'UTR and a 2658 bp open reading frame( ORF),encoding a peptide of 885 amino acids. The amino acid sequence of carp TLR5 gene shared 84. 46% identity with cirrhinus mrigala.

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What this paper is about

In this paper,we have isolated,sequenced and characterized the full-length cDNA of TLR5M gene from common carp by the methods of rapid amplification of cDNA ends( RACE) on the basis of its EST sequences. The results showed that the TLR5M gene was 3182 bp in length,vthe gene consists of a 38 bp 5' untranslated region( UTR),486 bp 3'UTR and a 2658 bp open reading frame( ORF),encoding a peptide of 885 amino acids. The amino acid sequence of carp TLR5 gene shared 84. 46% identity with cirrhinus mrigala.

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Available abstract

In this paper,we have isolated,sequenced and characterized the full-length cDNA of TLR5M gene from common carp by the methods of rapid amplification of cDNA ends( RACE) on the basis of its EST sequences. The results showed that the TLR5M gene was 3182 bp in length,vthe gene consists of a 38 bp 5' untranslated region( UTR),486 bp 3'UTR and a 2658 bp open reading frame( ORF),encoding a peptide of 885 amino acids. The amino acid sequence of carp TLR5 gene shared 84. 46% identity with cirrhinus mrigala.

Key concepts: Open reading frame, Complementary DNA, Biology, Untranslated region, Gene, Rapid amplification of cDNA ends, Genetics, Sequence analysis

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