Clonging and Sequence Analysis of Tumor Necrosis Factor Receptor-associated Factor 6 Full-length cDNA from Common Carp

The common carp tumor necrosis factor receptor-associated factor 6(TRAF 6)EST sequence was picked out from the cDNA library of peripheral blood leukocytes that was constructed.The cDNA library were separated from carp and stimulated with mitogen was screened by aprobe labeled with DIG.The full-length TRAF6 cDNA was cloned from recombinant phages.Sequence analysis indicated that it encompasses with a 25bp 5′-UTR and a 535bp 3′-UTR,the open reading frame(ORF)of which was 1632bp putatively coding 543 amino acids, and there were two motifs for mRNA instability ATTTA in the 3′-untranslated region.The predicted theoretical isoelectric point and molecular weight were 5.88 and 61.773ku,respectively.Its nucleotide homology with carp TRAF6 from GenBank was up to 99%.The protein sequence analysis showed that it shared typical sequence features of the TRAF family.