Up-regulated Expression of Hsp27 in Injured Spinal Cord Cells with Methylprednisolone Treatment in Early Period

Objective To study methylprednisolone′s(MP) effect of up-regulation of Hsp27 on provocation and enhancement of self-protection and self-repair to injured central nerves,and if the injured nerves can get protection in an early period,and therefore MP benefit injured central nerves′survival and regeneration. Methods The rats were randomly divided into two groups: acute spinal cord injury groups, acute spinal cord injury rats with MP treatment groups.Improved Allen′s weight drop model of acute spinal cord injury was prepared to study the pathological changes of spinal cord. 24 hours later,the spinal cords were taken out and cut,then the pathological changes of spinal cord cells with HE staining and the expression of Hsp27 in spinal cord cells with immunohistochemical staining were observed respectively. The data were analysed with SPSS soft ware.Results ①The expression of Hsp27 which is a protective to central nerves in the MP treatment groups was up-regulated. The quantity of Hsp27 in spinal cord cells in two groups has a difference in statistics (P0.01). ②The pathological changes of spinal cord cells were significantly improved in MP treatment groups.Conclusion The that high-dose methylprednisolone treatment in an early period induces the up-regulation of Hsp27 and it might provocate and enhance the self-protection and self-repair of the injured central nerves in view of morphology.