The Effect of Jiangu Granula on the Proliferation and PCNA、Cyclin D1 Genetic Expression in Osteoblast-like Cell in Vitro

Objective :To observate the effect of Jiangu granula on the proliferation and PCNA、Cyclin D1 Genetic expression in Osteoblast-like cell in vitro.Method: Preparation for serum containing Jiangu Granula and normal saline(NS),intervene in Osteoblast-like cell ROS17/2.8 for 48h and detect the proliferation by MTT method.Analyzed the cell cycle by flow cytometry(FCM) and calculated cells proliferation index(PI).To detect the expression levels of PCNA、Cyclin D1 mRNA by SYBR GREEN real-time polymerase chain reaction.Result: Rat of cell proliferation was the fastest when the concentration of serum containing Jiangu Granula was 20%.It was significantly faster than NS-group with the concentration between 10% and 30%.(P0.05).The cell percentage of G0/G1 phase of TCM-group was lower than NS-group significantly(P0.01).While the percentage of G2/M、S phase and PI were higher than NS-group significatantly with the same time(P0.01).The expression level of PCNA and Cyclin D1 mRNA of TCM-Group was higher than NS-group(P0.05).Conclusion:The serum containing Jiangu Granula stimulated OB in vitro to proliferate obviously,promoted the expression level of PCNA and Cyclin D1 mRNA.