Purification of total flavones from Abelmoschus L.Medic by macroporous resin

Aim To sieve the optimal purification of the total flavonoid from Abelmoschus L.Medic by exploring 5 types of macroporous resin.Methods The factors affecting separation,such as flow rate concentration and kind of eluting agent,were considered.Results Experiment results showed that D101 resin possesses high absorption capacity.The best craft was obtained as follows: the absorption and desorption rate was 1BV·h-1 and the content of the total flavonoid in liquid was 4.15 g·L-1.The desorption solvent was 70% ethanol and the resin can be regenerated three times.Conclusion The content of total flavones can be increased by 15 times by using the method.