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Optimal Conditions for Chitinase Production by Serratia Marcescens XJ-01

Zhen-yuan Nie

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Abstract

Objective: To determine the optimal conditions for chitinase production by Serratia marcescens XJ-01. Methods: The effects of culture conditions (e.g. carbon source, nitrogen source, temperature and pH) for chitinase production by Serratia marcescens XJ-01 were investigated by orthogonal experiment. Results: show that the optimal condition for producing chitinase is as follows: temperature of 32 ℃, pH 8.0, 5 g/L (NH4)2SO4 as the nitrogen source and 5 g/L colloidal chitin as the carbon source. Conclusions: The optimal conditions for chitinase production by Serratia marcescens were determined.

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What this paper is about

Objective: To determine the optimal conditions for chitinase production by Serratia marcescens XJ-01. Methods: The effects of culture conditions (e.g. carbon source, nitrogen source, temperature and pH) for chitinase production by Serratia marcescens XJ-01 were investigated by orthogonal experiment. Results: show that the optimal condition for producing chitinase is as follows: temperature of 32 ℃, pH 8.0, 5 g/L (NH4)2SO4 as the nitrogen source and 5 g/L colloidal chitin as the carbon source. Conclusions: The optimal conditions for chitinase production by Serratia marcescens were determined.

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Available abstract

Objective: To determine the optimal conditions for chitinase production by Serratia marcescens XJ-01. Methods: The effects of culture conditions (e.g. carbon source, nitrogen source, temperature and pH) for chitinase production by Serratia marcescens XJ-01 were investigated by orthogonal experiment. Results: show that the optimal condition for producing chitinase is as follows: temperature of 32 ℃, pH 8.0, 5 g/L (NH4)2SO4 as the nitrogen source and 5 g/L colloidal chitin as the carbon source. Conclusions: The optimal conditions for chitinase production by Serratia marcescens were determined.

Key concepts: Serratia marcescens, Chitinase, Chitin, Carbon source, Serratia, Microbiology, Food science, Nitrogen

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