In Vitro and in Vivo Behavior of Hydrothermally Prepared Hydroxyapatite Coating

Objective: To evaluate the biocompatibility of the hydrothermally prepared hydroxyapatite coating on titanium. Methods: The hydroxyapatite coating was hydrothermally prepared from CaHPO4 plasma sprayed coating. Cell culture was used for evaluate the cell biocompatibility of this new coating material, and then implanted into dog hip bone for histological evaluation of the interface. Results: The osteoblast could attach and proliferation on the HA coating material as usual. The levels of alkaline phosphatase activity produced by osteoblast were increased with the culture time. Ossointegration had been achieved on the coating surface after one month implanting. More bone were formed on the coating surface after three months implanting. The coating material was stable in vivo, and no solution or separation were found. Conclusion: The hydrothermally prepared hydroxyapatite coating on titanium has fine biocompatibility.