The protective effect of resveratrol on intestinal mucosal barrier in rats with severe acute pancreatitis

Objective To explore the protective effect of resveratrol(RES)on the intestinal injury in rats with severe acute pancreatitis(SAP).Methods Totally 72 SD rats were randomly divided into 3 groups:sham-operation(SO)group,SAP group and RES-treated(RES)group.The SAP models were induced by retrograde injection of 4% sodium taurocholate(0.1 mL/100 g)into biliary-pancreatic duct.In RES group,resveratrol(20 mg/kg)was infused immediately intravenously after SAP model was induced.Samples were obtained at 3,6 and 12 h after the induction of SAP model,respectively.The expression of intercellular adhesion molecular-1(ICAM-1)and vascular cell adhesion molecular-1(VCAM-1)were assessed by immunohistochemical staining.The serum TNF-α was detected by radioimmunity.The activities of superoxide dismutase(SOD)and malondialdehyde(MDA)in the small intestine were detected.Results Significantly decreased SOD(P0.05)and increased MDA(P0.05)in the intestine were observed after the induction of SAP at 3,6 and 12 h.The serum TNF-α level increased predominantly when compared with SO group(P0.05);the expression of ICAM-1 and VCAM-1 in the intestine increased in SAP group.RES could inhibit MDA in intestinal tissues and TNF-α level in serum,thus enhancing SOD activity.Conclusion RES can produce antioxidative effects,suppress the production of TNF-α as well as the expression of ICAM-1 and VCAM-1 in the intestine,thus protecting intestine mucosal barrier.