Immunophenotype analysis on 128 cases of acute lymphoblastic leukemia children

Aim:To study the immunophenotypical characteristics of acute lymphoblastic leukemia(ALL)and to investigate the complete remission and the prognosis of the different immunophenotypic ALL.Methods:Multiparameter flow cytometry and CD45/SSC gating were used to analyze the surface antigen expression in 128 cases of ALL and was compared with FAB subtype.The chemotherapies to all the ALL patients were carried out.Results:The results showed that the consistency rate of 128 patients was 81.25% between morphology and immunology,and 16 morphologic misdiagnosed cases were corrected by immunology.Lymphoid antigens were expressed in 104 of 128 patients with ALL and B or T lymphoid antigens were detected alone in 52 or 22 patients,respectively.And myeloid lineage-associated antigens were also found in ALL.30 patients with ALL showed aberrant myeloid antigens expression such as CD33 and CD13.Myeloid antigens were expressed alone in 4 ALL-L2 patients.8 patients were also diagnosed AML for myeloid antigens were expressed along with aberrant lymphoid antigens expression.8 patients were acute unidentified leukemia.4 patients with mixed acute leukemia showed lymphoid and myeloid antigens more than two kinds.In B-ALL CD19 was the most susceptible cluster designations.In T-ALL CD7 was the most sensitive cluster designations.Un-serial antigens including HLA-DR and CD34 had high positive expression rate.And complete remission(CR)rate of My+-ALL was significantly lower than that of My--ALL(χ2=17.26,P=0.028).Conclusion:Immunophenotype has diagnostic value to some special types of ALL children and it is the important complement to morphology.Multiparameter FCM could not only provide data of cell lineage and differentiation status but also detect phenotypic aberrancies,which is helpful for judging prognosis.