The protective mechanism of anisodamine on liver ischemia-reperfusion injury in rats

Objective To study the protective mechanism of anisodamine on liver ischemia-reperfusion injury in rats.Methods One hundreds and sixty male Wistar rats were randomly divided into the normal control group (n=10), ischemia-reperfusion group (n=50), physiologic saline group (n=50)and anisodamine group (n=50). The animals were killed at 1st,3rd, 6th,12th,24th h after ischemia induced for 60 min and followed by reperfusion. Plasma and(or) liver endothelin-1 (ET-1), hyaluronic acid (HA), glutamic-pyruvic transaminase enzyme (ALT) and malondialdehyde (MDA) were measured. Liver intracellular i and adenosine triphosphate (ATP) after being reperfused for 1 h were measured. Meanwhile, the histopathologic hepatic alterations were also observed.Results Plasma ET-1, HA, ALT and liver ET-1, MDA, intracellular i concentrations were markedly increased and liver ATP markedly decreased after liver ischemia-reperfusion. It was found that an intravenous injection of anisodamine (2.0 mg/kg) before ischemia-reperfusion could significantly decrease the levels of HA, ALT, MDA, intracellular i and increase ATP concentrations in plasma and(or) liver. Meanwhile, the histopathologic hepatic alterations were significantly lessened.Conclusions The anisodamine have protective effects on liver ischemia-reperfusion injury.