Study on blood clotting effect of different solvent extraction parts of Mume carbon

OBJECTIVE To study the blood clotting effect of different solvent extraction(petroleum ether,chloroform,ethyl acetate)of Mume carbon,and to pick up the optimal active extract parts;providing a basis for further separating active components research.METHODS Solvent extraction method was used to prepare solvent extraction of Mume carbon.Blood clotting time was determined by capillary method,bleeding time was measured by broken tail method in mice.RESULTS The bleeding time and blood clotting time of water decoction,chloroform and ethyl acetate extract parts of Mume carbon were shorter than that of negative control.Petroleum ether part and Mume carbon were no obvious difference compared with negative control.Blood clotting time and bleeding time of chloroform part was shorter than that of ethyl acetate part.CONCLUSION Mume and petroleum ether part have no obvious blood clotting effect.Mume carbon and chloroform,ethyl acetate parts have significant blood clotting effect.Chloroform part is the best active components.