The Effect of Ischemic Postconditioning on the Injury of Liver Function after Ischemia/Reperfusion in Rat Hind Legs

Objective To observe the effect of ischemic postconditioning on the injury of liver function after ischemia/reperfusion(I/R) in rat hind legs.Methods SD rat hind leg I/R model is set up with 32 healthy male SD rats randomly divided into 4 groups of 8.The hepatic function and the content changes of SOD,malondialdehyde and MPO in hepatic tissue were observed;the expressions of Bcl-2 and Bax in hepatic tissue were measured;the apoptosis in hepatic tissue was assessed.Results Compared with I/R,the activity of plasma enzyme and the concentrations of malondialdehyde and MPO in hepatic tissue in IPC and IPo decreased sharply(P0.05),while the concentrations of SOD increased significantly(P0.05).Moreover,the expression of Bcl-2 increased;the expression of Bax and the apoptotic index decreased,all of which are statistically different(P0.05).The difference between IPo and IPC was not obvious(P0.05).Conclusion IPo has obvious protective effects on the liver function after ischemia/reperfusion in rat hind legs.