Study on quality standard of Yukexin capsules

OBJECTIVE To establish a quality standard for Yukexin Capsules.METHODS Thin layer chromatography(TLC) and ultraviolet spectrophotometry(UV) were used for the identification of the content of the capsules,hyperoside in the capsules was determined using. RP-HPLC was used for the determination of hyperoside.The chromatographic procedure was carried out using Zorbax ODS C_(18)(250 mm×(4.6) mm,5 μm) as an analytical column and a mixture of 45 volume of methanol,55 volume of 5% phosphoric acid,(0.35) volume of triethylamine as a mobile phase at a flow rate of(1.0) mL·min~(-1).The detection wave length was set at 360 nm.RESULTS TLC chromatogram and UV spectrum of Yukexin capsule content were same as the hyperoside standard sample.The t_R of hyperoside was about4.9 minutes.Resolution between hyperoside and other peaks was more than (1.5) and its linear range was between(0.005) and(0.062 5) g·L~(-1) (r=(0.999 9)).The detection limit was(2.0) ng and the quontitation limit was (7.5) ng.The average recovery and RSD value of hyperoside were(98.91%) and(1.62%),respectively.CONCLUSION This method is sensitive and can be used for the quality control of Yukexin capsules.