NS398 induced apoptosis in renal cell carcinoma cells 786-O through a COX-2 dependent pathway

Objective To study the proliferative and apoptotic effects of NS398,a selective COX-2 inhibitor,on renal carcinoma cell line 786-O and its possible mechanisms.Methods 786-O cells were treated with different concentrations of NS398 for 24h,48h,72h and 96h.The proliferation of 786-O cells was studied by MTT assay.The expression of COX-2 mRNA was analyzed by RT-PCR.Immuno cytochemistry was used to detect the expressions of COX-2.The release levels of PGE2 was measured by enzyme-linked immunosorbent assay(ELISA).Flow cytometric analysis was used to detect the apoptosis status of 786-O cells.Results NS398 resulted in significant antiproliferative effects on 786-O cells in dose and timedependent.RT-PCR showed the expressions of COX-2 were significantly inhibited by NS398.Immunocytochemistry showed the expressions of COX-2 were signifIcantly inhibited by NS398.The release levels of PGE2 were decreased compared with those of the control group.Apoptosis rate was(31.5±2.1)% and(14.3±1.4)% in the 786-O cells treated with NS398 in different concentrations of 180μmol/L and 30μmol/L,which was significantly higher than that in the control group(2.1±0.4)%.Conclusion NS398 can inhibit proliferation of renal carcinoma cell line 786-O and induce its apoptosis in vitro,which may contributed to the COX-2 dependent pathway.