Effects of NS-398 on the proliferation and apoptosis of human cervical carcinoma HeLa cell

Objective To investigate the effects and mechanism of COX-2 selective inhibitor NS-398 on the human cervical carcinoma cell line HeLa.The possibility of treatment by NS-398 was also discussed.Methods The effects of NS-398 on the proliferation of HeLa cells was assessed by Cell Counting Kit-8(CCK-8)method.The changes of cell cycle distribution was analyzed by flow cytometry with PI staining;Annexin Ⅴ-FITC/PI double-staining method was used to detect early apoptosis.Results The proliferation of HeLa cells was significantly inhibited by NS-398.The inhibitory rates increased obviously with concentration increasing or time prolonging.Flow cytometry demonstrated that G1 phase cells decreased,while the S-phase cells increased significantly in NS-398 treatment group,the difference was significant compared with control group(P0.05);After treated by NS-398 for 24 hours,the apoptosis rates were 5.60%,12.89%,27.03% in 10 μmol/L group,20 μmol/L group and 40 μmol/L group,respectively,while apoptosis didn't be detected in the control group.Conclusions NS-398 significantly inhibited the growth of cervical carcinoma cells in a dose-dependent and time-dependent manner.The effects of NS-398 in cervical cancer cells may due to the induction of apoptosis.