Primary exploration of peptide nucleic acid chip techniques

Objective To deal with the questions in oligonucleotide chip,PNA probes were subsituted for DNA probes and PNA chips were prepared.By hybridizing with the ODN and HBV-DNA PCR fragment,the hybridizing conditions of PNA chip were discussed. Methods Both the DNA chip and the PNA chip were used to detect the single-base mutation.And the PNA chips also were used to detect HBV-DNA.Results Capability of recognization of PNA probed were stronger than those of DNA when they were used to detect the single-base mutation.The results about detection of HBV-DNA were identical when a kit was used.Conclusion The unique structure of PNA decides the advantages in hybridization such ad the high stability and strong speciality.