The Research of the Culture Condition of Amylase Produced by Bacterial Strain Optimized by the Method of the Response Surface Analyze Techniques

In the single-factor test,high-yielding strains of amylase culture conditions were optimized based on the response surface analysis method.Through single factor test,the effects on produced amylase of the different temperature,pH,time,carbon source concentration,nitrogen concentration,and shaker speed were concluded.After the central composite design of peptone concentration,the concentration of corn flour,pH and temperature and other major factors,got the result from the response surface analysis that the pH effect on enzyme production significantly,the best culture conditions for pH=6.5,the concentration of 0.3% corn meal,the concentration of peptone 0.5%,incubation temperature of 35 ℃,the culture time of 30 hours.