Investigation on bacterial flora and analysis of their antibiotic resistance from burn patients

Objective To investigate the bacterial flora from burn patients and their antibiotic resistance in our department, so as to provide reference for clinical application in the treatment of burn patients. Methods Four hundred and thirty-one strains of bacteria were isolated from wounds, blood and urine specimens of the burn patients hospitalized in our department from January, 2002 to December, 2004, and among them 5 strains were analyzed. Disc agar dilution method was used for determination of drug sensitivity. Results Among all bacteria. Cram negative(G-) bacilli accounted for 71% , and Gram positive(G+) cocci accounted for 29%. The five predominant bacteria from the wounds were Pseudomonas aeruginosa(36% ) , Staphylococcus aureus (SA, 17% ), Escherichia coli( 15% ), Aerobacter cloacae(8% ) , Staphylococcus epidermidis(Se, 7% ). Among all the G- bacilli, Pseudomonas aeruginosa accounted for 50.7% , 90.0% of them were drug resistant, while Staphylococcus aureus accounted for 60% of all G+ cocci, and MRSA was identified in 91.9% of Staphylococcus aureus, which was only sensitive to Vancomycin. Aerobacter cloacae were sensitive to Imipenem and Cefoperazone/Sulbactam. In general, Vancomycin , Imipenem and Cefoper-azone/Sulbactam were effective to the bacteria. Conclusion G- bacilli were still dominant in our burn ward. The high antibiotic resistance may be related to the production of MRSA and β-lactamase, especially with a high incidence of ESBL producing bacteria.