Relationship between S100A4 mRNA expression and differentiation and metastasis of renal cell carcinoma

Objective To study the relationship between S100A4 mRNA expression level and differentiation and metastasis of renal cell carcinoma combined clinical data and pathology together. Methods This study was based on tissues from 31 patients with renal cell carcinoma treated surgically from 2001 to 2004. After RT-PCR amplification on 31 RCC tissues and paired normal kidney tissues besides the tumor, we detected fluorescence dosage of S100A4 mRNA in tissues, and we obtained S100A4 mRNA gene fragments(212 bp). Results S100A4mRNA expression was detected in all of the RCC tissues and para-carcinoma tissues using fluorescence. Expression of 29 RCC tissues′ S100A4 mRNA was higher than paired para-carcinoma tissues. Of all cases, patients of low-differentiation RCC had higher expression rate than high-differentiation RCC obviously(7.94 vs 5.06,P 0.001); patients of metastasis also had higher expression rate than non-metastasis(9.61 vs 5.53,P 0.001). There was no clearly difference of expression rate between Granular cell carcinoma and Clear cell carcinoma(6.98 vs 6.02,P 0.05). Conclusions S100A4 mRNA is correlated to RCC differentiation and may play an important role in prognosis of RCC. Differential expression of S100A4 mRNA is correlated to metastasis of RCC. S100A4 may be one of markers on assessment of patient′s condition developing and clinical treatment instruction in RCC.