2011•China Medical HeraldRequires access
An improved method for isolation of hepatic stellate cells
Shourong Shen
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Abstract
Objective:To investigate a simple and efficient method for isolation of hepatic stellate cells(HSC),this may provide raw material for studying the liver fibrosis at the cellular level.Methods:Cell suspension and Nycodenz cell separating medium were mixed,and the concentration of the fluid was adjusted to be 11.3%(w/v),HSC were isolated by momolayer gradient centrifugation method.The cells viability was identified by Trypan blue exclusion staining.The purity of HSCs was identified by the expression of desmin immunocytochemistry.Results:The yield rate was about(4.0±0.5)×107 per rat,the survival and the purity rate of HSC were 90%,95% respectively.Conclusion:The simple and efficient method is an ideal one for the isolation and culture of rat HSCs,which is worth spreading.