2005•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access
Isolation,identification and culture of rat hepatic stellate cells
Qiu Zhao
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Abstract
To improve a convenient method of isolating and culturing rat hepatic stellate cells (HSCs). Rat hepatic stellate cells (HSCs) were isolated by the following steps: intubating portal vein, injecting diluted heparin, liver perfusion, collagenase liver circulating perfusion in vitro, cells filtering, density gradient centrifugation. But the course of liver cells digestion by pronase E was left out. The cells viability was identified by Trypan blue exclusion staining. The purity of HSCs was identified by the expression of desmin immunocytochemistry method. The yield rate of HSCs was 0.5-1.0×107 per rat . The purity was (92.2±2.0)%.The cells viability was (97.8±0.3)%. [Conclusions] The modified method of isolating and culturing HSC is simple,cheap , reliable and worth further research.