2012•Zhongguo redai yixueRequires access
Inhibiting effect and basic mechanism of salvianolic acid B on mouse hepatoma hepa1-6 cell line
Ge WenHua
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Abstract
Objectives To observe the inhibiting effect and basic mechanism of Salvianolic acid B on mouse hepatoma hepa1-6 cell line. Methods Hepa1-6 cell culture was taken as in vitro model.MTT method was used for testing the cytotoxicity effect of Salvianolic acid B on the hepatoma cell line.Hepatoma cell death style was tested with Annexin V method in flow cytometry.Apoptosis related genes were tested with realtime quantitative PCR assay. Results Salvianolic acid B have marked cytotoxic effect on the heap1-6 cell line in certain range of concentration.Its TC50 is 31.9ug/ml.The proportion of hepa1-6 cell apoptosis were between 27.58 and 74.26% when Salvianolic acid B was administrated in the concentration of 25-50ug/ml.The anti-apoptosis factor BCL-2 mRNA level was significantly decreased and pro-apoptosis factor Bax mRNA level was significantly elevated. Conclusion Salvianolic acid B can significantly inhibit the propagation of hepa1-6 cell line in vitro.The related mechanism is induction of cell apoptosis.