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The Extraction, Purification and Content Determination of Polysaccharide from Flos Lonicerae Japonicae

Guanghai Liu

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Abstract

Objective To extract and purify polysaccharide of Ligustrum lucidum and determine carbohydrate content. Methods The crude polysaccharide was obtained by hot water extraction and ethanol precipitation. The crude polysaccharide was deproteinized by Sevag method.The relative percentage content of Flos Lonicerae polysaccharide was studied with sulfuric acid-phenol method. Results The carbohydrate content of crude polysaccharide and purified polysaccharide was 61.27% and 80.54%. Conclusion A purified polysaccharide was obtained from Flos Lonicerae Japonicae.

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Objective To extract and purify polysaccharide of Ligustrum lucidum and determine carbohydrate content. Methods The crude polysaccharide was obtained by hot water extraction and ethanol precipitation. The crude polysaccharide was deproteinized by Sevag method.The relative percentage content of Flos Lonicerae polysaccharide was studied with sulfuric acid-phenol method. Results The carbohydrate content of crude polysaccharide and purified polysaccharide was 61.27% and 80.54%. Conclusion A purified polysaccharide was obtained from Flos Lonicerae Japonicae.

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Available abstract

Objective To extract and purify polysaccharide of Ligustrum lucidum and determine carbohydrate content. Methods The crude polysaccharide was obtained by hot water extraction and ethanol precipitation. The crude polysaccharide was deproteinized by Sevag method.The relative percentage content of Flos Lonicerae polysaccharide was studied with sulfuric acid-phenol method. Results The carbohydrate content of crude polysaccharide and purified polysaccharide was 61.27% and 80.54%. Conclusion A purified polysaccharide was obtained from Flos Lonicerae Japonicae.

Key concepts: Flos, Polysaccharide, Chemistry, Chromatography, Ethanol precipitation, Hot water extraction, Extraction (chemistry), Content determination

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