Extraction and identification of mangiferin from Mangifera indica leaves

AIM: To explore the extractive and identifing methods of mangiferin extracted from domestic Mangifera indica leaves. METHODS: Mangiferin was extracted and purificated from Mangifera indica leaves by alcohol percolation and other methods, and identified by polyamide thin layer chromatography, spectral absorption scan analysis, infra-red absorption spectrum analysis, high performance liquid chromatography and high performance capillary electrophoresis methods RESULTS: The extractive mangiferin was golden yellow. It showed as needle style crystal under light microscopy examination. Its melting point was 271-273 ℃. Ferric trichloride and stroutium chloride reactions were positive. The Rf value of polyamide thin layer chromatography was 0.46. Spectrum absorption,infra-red spectrum, high performance liquid chromatography, high performance capillary electrophoresis results and all parameters above were similar to that of the control. CONCLUSIONS: The purity of mangiferin extracted from domestic Mangifera indica leaves is similar to that of the standard confirmed by above determined methods. It can be used in pharmacology research.