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Research on Contents of Mangiferin in Different Part of Mangifera Indica

Wang Ning

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Abstract

To establish the contents of mangiferin in mangifera indica by HPLC,the analysis of HPLC was carried out with Waters C18 column(4.6mm×150mm,5μm).The mobile phase was CH_3OH-0.04%H_3PO_4(28∶72)with the flow-rate at 1.0 mL/min.The wave-length was 258nm with room temperature.Experiment results show that the liner range of mangiferin is 0.008 25～0.016 5μg(r=0.999 6),the average recovery is 98.48% and RSD is 2.65%.The method is simple and fast,it has a good linear relationship and could be used for the contents detection of mangiferin in mangifera indica.

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What this paper is about

To establish the contents of mangiferin in mangifera indica by HPLC,the analysis of HPLC was carried out with Waters C18 column(4.6mm×150mm,5μm).The mobile phase was CH_3OH-0.04%H_3PO_4(28∶72)with the flow-rate at 1.0 mL/min.The wave-length was 258nm with room temperature.Experiment results show that the liner range of mangiferin is 0.008 25～0.016 5μg(r=0.999 6),the average recovery is 98.48% and RSD is 2.65%.The method is simple and fast,it has a good linear relationship and could be used for the contents detection of mangiferin in mangifera indica.

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Available abstract

To establish the contents of mangiferin in mangifera indica by HPLC,the analysis of HPLC was carried out with Waters C18 column(4.6mm×150mm,5μm).The mobile phase was CH_3OH-0.04%H_3PO_4(28∶72)with the flow-rate at 1.0 mL/min.The wave-length was 258nm with room temperature.Experiment results show that the liner range of mangiferin is 0.008 25～0.016 5μg(r=0.999 6),the average recovery is 98.48% and RSD is 2.65%.The method is simple and fast,it has a good linear relationship and could be used for the contents detection of mangiferin in mangifera indica.

Key concepts: Mangiferin, Mangifera, Chromatography, Chemistry, High-performance liquid chromatography, Linear relationship, Linear range, Mathematics

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