Inhibitory effect of expression of c-myc antisense RNA on human hepatocarcinoma cell line HepG2 in vitro

Objective:To study the inhibitory effects of c-myc antisense RNA on human hepatocarcinoma cells HepG2 in vitro.Methods:Three days after the four element complex with c-myc antisense RNA for transient transfection,the expression of c-myc protein,cell cycle and cell apoptosis were assayed by flow cytometry.Established hepato carcinoma cell line which could express c-myc antisense RNA stably.By growth curve, to study the biological effects on HepG2/as-c-myc cells which could express c-myc antisense RNA stably.Results:c-myc antisense RNA caused the expression of c-myc protein of HepG2 cells diminish;induced cell apoptosis and effected cell cycle pause at G0/G1 phase.Study of HepG2/as-c-myc cells,which could express c-myc antisense RNA stably,revealed that c-myc antisene RNA could inhibit cell growth.Conclusion:c-myc antisene RNA could effectively reduce the expression of c-myc protein on human hepatocarcinoma cells HepG2,induce cell cycle pause at G0/G1 phase and inhibit the growth of hepatocarcinoma cells.